Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports

Authors:  Oscar Fernández, Isabel Díaz, Carlos F. Torres, Montserrat Tobajas, Víctor Tejedor, Rosa M. Blanco

Abstract:
The use of immobilized enzymes as catalysts may be limited by particle size which must be larger than the mesh that retains them in the reactor. Octyl-silica (OS) beads of 70 m average size were agglomerated to obtain hybrid organic–inorganic composites with particle sizes between 100 and 200 m. The agglomeration process has been achieved by polymerization of methacrylate from glycidyl methacrylate and ethylene dimethacrylate in the presence of silica beads and further functionalization ofthe composite with octyl groups.
        Methacrylate content of the composite (20%) is high enough to stick OS beads, and low enough to preserve the advantages ofthese particles as supports. The properties ofthe octyl silica particles for lipase immobilization have been very closely reproduced with the octyl-silica-methacrylate (OSM) composite. Enzyme loading of 210 mg lipase per gram of support has been achieved on OSM vs 230 mg/g on OS. Also catalytic activity values are close for both catalysts, OSM-lipase remaining fully active and stable after 15 cycles in acetonitrile.

Keywords:
Enzyme immobilization
Particle agglomeration
Hydrophobic supports
Diffusional limitations
Hybrid composites

Published in: Applied catalysis A: General  (Volume 450, Pages 1-274, 15 January 2013)

Publisher: Elsevier

ISSN Information: 0926-860X

Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports

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